Hi all, I'm having problems with my PiCamera being unable to take consistent timelapse images.
I'm using a V1 Pi Camera to take a timelapse of fluorescence occurring in a well plate. You can see an example of such an image below. I need the images to remain as consistent as possible to detect small changes in colour of the wells, so I've followed the instructions on the Picamera documentation page and used the same settings as they did. I did an image capture every 2 min for about 7 hours.
I analyzed the colour content of the timelapse images and noticed that the brightness was gradually dipping during the first 100 mins of the footage. I did a diagnostic timelapse where I kept everything constant, yet I still saw the dip, consistently ending at the 100 min mark. You can see the dip in brightness over time in the graph I've attached below, it represents the average RGB values of the images over the course of the diagnostic timelapse. I've isolated all possible causes including the illumination source and the colour of the plate, and I think the culprit is very likely the camera itself.
I'm not sure if there's some Picamera setting I've missed out , or if its due to some kind of distortion from the camera heating during use.
Has anyone experienced this issue before and can advise?